Interferon production and protein synthesis in chick cells.

Interferon production in chick-embryo cells. The effect of puromycin and p-fluorophenylalanine.

1. When chick-embryo cells were treated with ultraviolet-inactivated influenza virus (Melbourne strain), interferon was produced after a lag period of about 10hr. 2. The addition of small amounts of either puromycin or p-fluorophenylalanine immediately after the virus inhibited the subsequent production of interferon. Both inhibitors primarily affected protein synthesis, and it is concluded tha...

The Effect of Beta Interferon on Dendritic Cells and Cytokine Synthesis by CD4+ T Cells

Background: Dendritic cells (DC) are a key regulator of the immune response, and interferon- beta (IFN-β) is considered an immunomodulatory molecule for DC. Objective: The purpose of this study was to evaluate the ability of IFN-β treated DC to induce cytokine secretion by CD4+ T cells. Methods: Dendritic cells were generated from blood monocytes with granulocyte-monocyte colony-stimulating fac...

Effect of Activation and Inhibition of Cellular PKR on Coxsackievirus B3 Replication

The ds-RNA activated protein kinase (PKR) is a serine-threonine kinase with MW of 68 KDa. It belongs to a family of kinases that control one of the translational initiation factors, eIF2. PKR is produced at high level in response to viral infection. This protein by phosphorylating eIF2 inhibits cellular protein synthesis. In this study, the effect of gamma interferon (IFN-γ), an activator, and ...

Interferon production and viral ribonucleic acid synthesis in chick embryo cells.

Interferon effect on cellular functions: enhancement of virus induced inhibition of host cell protein synthesis in interferon-treated cells.

Total protein synthesis in mouse cells but not in confluent chick embryo fibroblasts (CEF) is inhibited shortly after infection with vaccinia virus. This inhibition by the infecting virus is enhanced drastically if the mouse cells have been pretreated with homologous interferon pre­ parations. The enhanced reduction of protein synthesis also occurs if the cells are treated with actinomycin D an...